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KMID : 0811720140180020103
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Korean Journal of Physiology & Pharmacology
2014 Volume.18 No. 2 p.103 ~ p.108
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Push-in Head Restraining Apparatus for Intracranial Self Stimulation Tasks in Rats
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Roh Moo-Taek
Jang Il-Sung
Lee Maan-Gee
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Abstract
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Head restraining is an experimental technique that firmly secures the animal’s head to a fixation apparatus for the precise control and sensing of behaviors. However, procedural and surgical difficulties and limitations have been obstructing the use of the technique in neurophysiological and behavioral experiments. Here, we propose a novel design of the head-restraining apparatus which is easy to develop and convenient for practical use. Head restraining procedure can be completed by sliding the head mounter, which is molded by dental cement during implantation surgery, into the port, which serves as matching guide rails for the mounter, of the fixation bar. So neither skull-attached plates nor screws for fixation are needed. We performed intracranial self stimulation experiment in rats using the newly designed device. Rats were habituated to acclimatize the head-restraint environment and trained to discriminate two spatially distinguished cues using a customized push-pull lever as an operandum. Direct electrical stimulation into the medial forebrain bundle served as reward. We confirmed that head restraining was stable throughout experiments and rats were able to learn to manipulate the lever after successful habituation. Our experimental framework might help precise control or sensing of behavior under head fixed rats using direct electrical brain stimulation as a reward.
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KEYWORD
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Apparatus, Head fixation, Intra-cranial self-stimulation, Operant conditioning, Rat
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